Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Applied Biosystems™ ViralSEQ™ dPCR Lentivirus Physical Titer Kit
The Applied Biosystems ViralSEQ dPCR Lentivirus Physical Titer Kit is a one-step digital PCR (dPCR) assay for the reproducible quantitation of lentiviral particles in supernatants from cell-based bioproduction systems.
Applied Biosystems™ SteriSEQ™ Rapid Sterility Testing Kit
The SteriSEQ Rapid Sterility Testing Kit is a TaqMan-based quantitative PCR (qPCR) kit for the detection of bacteria and fungi in complex bioproduction samples.
Applied Biosystems™ resDNASEQ™ Quantitative Vero DNA Kits
The resDNASEQ Quantitative Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines.
Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay
Rapidly detect E. coli O157:H7, stx, eae, in food samples in as little as 10 hours with highly-specific, simple to use real-time PCR Thermo Scientific™ SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay.
Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR Starter Kit
The QuantStudio Absolute Q Digital PCR Starter Kit is designed to confirm the performance of the QuantStudio Absolute Q Digital PCR System either at the time of system installation or as part of future, user-defined system verification procedures.
Applied Biosystems™ MycoSEQ™ Plus Mycoplasma Detection Kits
The MycoSEQ™ Plus Mycoplasma Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.
Applied Biosystems™ PureQuant Monocyte Assay
The Applied Biosystems PureQuant assays are a family of assays that accurately identify and quantify specific immune cell types in a mixed population of cells. These assays are TaqMan qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier. The assays include standards and controls and each 96-well plate can accommodate up to seven test samples.
Applied Biosystems™ TaqCheck™ SARS-CoV-2 Control Dilution Buffer
Applied Biosystems TaqCheck SARS-CoV-2 Control Dilution Buffer is required for use of the TaqCheck SARS-CoV-2 Fast PCR Assay. It is used to dilute the RNA control.
Applied Biosystems™ resDNASEQ™ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kits
The resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit is a quantitative PCR (qPCR)-based system for the detection of residual kanamycin-resistant plasmids.
Applied Biosystems™ resDNASEQ™ dPCR E1A DNA Fragment Length Kit
The resDNASEQ dPCR E1A DNA Fragment Length Kit is an absolute quantitation PCR-based system that measures the fragment lengths of residual host-cell DNA by targeting the E1A gene in HEK 293 cell lines, which are predominantly used in the development of gene therapies, cell-based vaccines, and similar biotherapeutics.
Thermo Scientific™ SureTect™ Cronobacter species PCR assay
Simply and accurately detect Cronobacter species in low or large sample sizes of powdered infant formula, and related ingredients, and production environment samples in less than 20 hours with the Thermo Scientific™ SureTect™ Cronobacter Species PCR Assay.
Thermo Scientific™ SureTect™ Brettanomyces spp. and Saccharomyces cerevisiae Multiplex PCR Assay
Rapidly detect and differentiate Brettanomyces spp. and S. cerevisiae in concentrate, semi-finished products, and environmental samples with the Thermo Scientific™ SureTect™ Brettanomyces spp. and S. cerevisiae Multiplex PCR Assay.
Applied Biosystems™ TrueMark™ Enteric Bacterial Select Panel I Combo Kit
The TrueMark Enteric Bacterial Select Panel I Combo Kit includes the TrueMark Enteric Bacterial Select Panel I and TrueMark Infectious Disease 1‐Step Multiplex Master Mix for simplified ordering.
Applied Biosystems™ resDNASEQ™ Quantitative Sf9 and Baculovirus DNA Kits
The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform.
Applied Biosystems™ TaqCheck™ SARS-CoV-2 Control
The Applied Biosystems™ TaqCheck™ SARS-CoV-2 Control is a synthetic RNA positive control that contains target sequences for the SARS-CoV-2 S and N gene assays included in the TaqCheck™ SARS-CoV-2 Fast PCR Assay.